Overexpressed in Cisplatin-resistant Human Cancer Cell Lines with Decreased Drug Accumulation'
نویسندگان
چکیده
By targeting the AlP binding conserved domain in three AlP binding cassette superfamily proteins (P-glycoprotein, multidrug resistance pro tein, and cystic fibrosis transmembrane regulator), we isolated the cDNA of a new AlP binding cassette superfamily that was specifically enhanced in a cisplatin-resistant human head and neck cancer KB cell line. A human clone homologous to rat cnnalicular multispecific organic anion trans. porter (cMOAT) was found and designated human cMOAT. Fluorescence in situ hybridization demonstrated the chromosomal locus of the gene on chromosome 10q24. The human cMOAT eDNA hybridized a 6.5-kb mRNA that was expressed 4to 6-fold higher by three cisplatin-resistant cell lines derived from various human tumors exhibiting decreased drug accumulation. Human cMOAT may function as a cellular cisplatin trans porter.
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